Selected publications

Aging retinal pigmented epithelium: omics-based insights into vision decline

Matei IV and Paraoan L (2024)

Aging (Albany NY) 16(12):10201-10202. doi: 10.18632/aging.205914.

Fucoxanthin diminishes oxidative stress damage in human placenta-derived mesenchymal stem cells through the PI3K/Akt/Nrf-2 pathway.

Suwanmanee G, Tantrawatpan C, Kheolamai P, Paraoan L, Manochantr S. (2023)

Sci Rep (Nature Publishing) 13(1):22974. doi: 10.1038/s41598-023-49751-5.

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/38151503/

Carlsson E, Sharif U, Supharattanasitthi W and Paraoan L. (2023)

Analysis of Wild Type and Variant B Cystatin C Interactome in Retinal Pigment Epithelium Cells Reveals Variant B Interacting Mitochondrial Proteins

Cells 12(5):713. doi: 10.3390/cells12050713.

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/36899848/

Dhirachaikulpanich D, Lagger C, Chatsirisupachai K, de Magalhães JP, Paraoan L. (2022)

Intercellular communication analysis of the human retinal pigment epithelial and choroidal cells predicts pathways associated with aging, cellular senescence and age-related macular degeneration.

Front Aging Neurosci. 14:1016293. doi: 10.3389/fnagi.2022.1016293

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/36408112/

Saptarshi N, Porter LF, Paraoan L. (2022)

PERK/EIF2AK3 integrates endoplasmic reticulum stress-induced apoptosis, oxidative stress and autophagy responses in immortalised retinal pigment epithelial cells.

Sci Rep. 12(1):13324. doi: 10.1038/s41598-022-16909-6

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/35922637/

PERK downregulation in RPE cells impairs ER-stress induced apoptosis in a Bax/Bcl-2-dependent manner.
PERK downregulation in RPE cells impairs ER-stress induced apoptosis in a Bax/Bcl-2-dependent manner.

Jantalika T, Manochantr S, Kheolamai P, Tantikanlayaporn D, Saijuntha W, Pinlaor S, Chairoungdua A, Paraoan L, Tantrawatpan C. (2022)

Human chorion-derived mesenchymal stem cells suppress JAK2/STAT3 signaling and induce apoptosis of cholangiocarcinoma cell lines.

Sci Rep. 12(1):11341. doi: 10.1038/s41598-022-15298-0

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/35790790/

Mahmud NM, Paraoan L, Khaliddin N, Kamalden TA. (2022)

Thymoquinone in Ocular Neurodegeneration: Modulation of Pathological Mechanisms via Multiple Pathways.

Front Cell Neurosci. 16:786926. doi: 10.3389/fncel.2022.786926. eCollection 2022.PMID: 35308121

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/35308121/

Saptarshi N, Green D, Cree A, Lotery A, Paraoan L, Porter LF. (2021)

Epigenetic Age Acceleration Is Not Associated with Age-Related Macular Degeneration.

Int J Mol Sci. 22(24):13457. doi: 10.3390/ijms222413457.PMID: 34948253

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/34948253/

Butler JM, Supharattanasitthi W, Yang YC, Paraoan L. (2021)

RNA-seq analysis of ageing human retinal pigment epithelium: Unexpected up-regulation of visual cycle gene transcription.

J Cell Mol Med. 25(12):5572-5585. doi: 10.1111/jcmm.16569. Epub 2021 May 1.PMID: 33934486

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/33934486/

RNA-seq analysis of ageing human retinal pigment epithelium. Reactome pathway analysis identifies an unexpected set of visual cycle-related genes that have increased expression with increasing age. (Significantly positively correlated genes (SCG), and complexes which include a SCG, highlighted in green.)
RNA-seq analysis of ageing human retinal pigment epithelium. Reactome pathway analysis identifies an unexpected set of visual cycle-related genes that have increased expression with increasing age. (Significantly positively correlated genes (SCG), and complexes which include a SCG, highlighted in green.)

Muangnoi C, Phumsuay R, Jongjitphisut N, Waikasikorn P, Sangsawat M, Rashatasakhon P, Paraoan L, Rojsitthisak P. (2021)

Protective Effects of a Lutein Ester Prodrug, Lutein Diglutaric Acid, against H2O2-Induced Oxidative Stress in Human Retinal Pigment Epithelial Cells.

Int J Mol Sci. 22(9):4722. doi: 10.3390/ijms22094722.PMID: 33946898

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/33946898/

Chatsirisupachai K, Lesluyes T, Paraoan L, Van Loo P, de Magalhães JP. (2021)

An integrative analysis of the age-associated multi-omic landscape across cancers.

Nat Commun. 12(1):2345. doi: 10.1038/s41467-021-22560-y.PMID: 33879792

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/33879792/

Roberts O, Paraoan L. 

PERP-ing into diverse mechanisms of cancer pathogenesis: Regulation and role of the p53/p63 effector PERP.

Biochim Biophys Acta Rev Cancer. 2020 Aug;1874(1):188393. doi: 10.1016/j.bbcan.2020.188393.

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/32679166/  

Known and hypothesised effects of downregulation of the apoptosis effector PERP in cancer cells.
Known and hypothesised effects of downregulation of the apoptosis effector PERP in cancer cells.

Dhirachaikulpanich D, Li X, Porter LF, Paraoan L.

Integrated Microarray and RNAseq Transcriptomic Analysis of Retinal Pigment Epithelium/Choroid in Age-Related Macular Degeneration.

Front Cell Dev Biol. 2020 Aug 21;8:808. doi: 10.3389/fcell.2020.00808 

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/32984320/ 

Paraoan L, Sharif U, Carlsson E, Supharattanasitthi W, Mahmud NM, Kamalden TA, Hiscott P, Jackson M, Grierson I.

Secretory proteostasis of the retinal pigmented epithelium: Impairment links to age-related macular degeneration.

Prog Retin Eye Res. 2020 Apr 9:100859. doi: 10.1016/j.preteyeres.2020.100859.

View abstract on Pubmed link: https://pubmed.ncbi.nlm.nih.gov/32278708/

Carlsson E, Supharattanasitthi W, Jackson M & Paraoan L.

Increased Rate of Retinal Pigment Epithelial Cell Migration and Pro-Angiogenic Potential Ensuing From Reduced Cystatin C Expression

Invest Ophthalmol Vis Sci. 2020 Feb 7;61(2):9. doi: 10.1167/iovs.61.2.9.

View abstract on Pubmed link:https://www.ncbi.nlm.nih.gov/pubmed/32049341 

McDonnell S.J., Spiller D.G., White M.R.H., Prior I. & Paraoan L.

ER stress-linked autophagy stabilizes apoptosis effector PERP and triggers its co-localization with SERCA2b at ER–plasma membrane junctions

Cell Death Discovery (2019) 5:132; doi:10.1038/s41420-019-0212-4

View abstract on Pubmed link: https://www.ncbi.nlm.nih.gov/pubmed/31508245

ER stress-modulated increased interaction of PERP and SERCA2b at ER-plasma membrane points of contact

ER stress-modulated increased interaction of PERP and SERCA2b at ER-plasma membrane points of contact

Muangnoi C, Ratnatilaka Na Bhuket P, Jithavech P, Supasena W, Paraoan L, Patumraj S, Rojsitthisak P.

Sci Rep. 2019 Aug 12;9(1):11718. doi: 10.1038/s41598-019-48124-1.

Curcumin diethyl disuccinate, a prodrug of curcumin, enhances anti-proliferative effect of curcumin against HepG2 cells via apoptosis induction.

Supharattanasitthi W, Carlsson E, Sharif U, Paraoan L.

CRISPR/Cas9-mediated one step bi-allelic change of genomic DNA in iPSCs and human RPE cells in vitro with dual antibiotic selection.

Sci Rep 2019 9:174. doi: 10.1038/s41598-018-36740-2.

View abstract on Pubmed link: https://www.ncbi.nlm.nih.gov/pubmed/30655567

Stem Cells

Pluripotent stem cells following first gene editing of AMD risk factor (Sci Rep 2019 9:174)

Muangnoi C, Sharif U, Ratnatilaka Na Bhuket P, Rojsitthisak P, Paraoan L.

Protective Effects of Curcumin Ester Prodrug, Curcumin Diethyl Disuccinate against H2O2-Induced Oxidative Stress in Human Retinal Pigment Epithelial Cells: Potential Therapeutic Avenues for Age-Related Macular Degeneration.

Int J Mol Sci. 2019 Jul 9;20(13). pii: E3367. doi: 10.3390/ijms20133367.

View abstract on Pubmed link:https://www.ncbi.nlm.nih.gov/pubmed/31323999 

Porter LF, Saptarshi N, Fang Y, Rathi S, den Hollander AI, de Jong EK, Clark SJ, Bishop PN, Olsen TW, Liloglou T, Chavali VRM, Paraoan L.

Whole-genome methylation profiling of the retinal pigment epithelium of individuals with age-related macular degeneration reveals differential methylation of the SKI, GTF2H4, and TNXB genes.

Clin Epigenetics. 2019 11(1):6. doi: 10.1186/s13148-019-0608-2.

View abstract on Pubmed link: https://www.ncbi.nlm.nih.gov/pubmed/30642396

Sharif U, Mahmud NM, Kay P, Yang YC, Harding SP, Grierson I, Kamalden TA, Jackson MJ, Paraoan L.

Advanced glycation end products-related modulation of cathepsin L and NF-κB signalling effectors in retinal pigment epithelium lead to augmented response to TNFα.

Epub 2018 Oct 19. J Cell Mol Med. 2019 23(1):405-416. doi: 10.1111/jcmm.13944. Epub 2018 Oct 19.

View abstract on Pubmed link: https://www.ncbi.nlm.nih.gov/pubmed/30338926

Butler JM, Hall N, Narendran N, Yang YC, Paraoan L.

Identification of candidate protective variants for common diseases and evaluation of their protective potential.

BMC Genomics. 2017 18(1):575. doi: 10.1186/s12864-017-3964-3. 

View abstract on Pubmed

Awais, Spiller, White, Paraoan L. p63 is required beside p53 for PERP-mediated apoptosis in uveal melanoma. Br J Cancer. 2016 Sep 1. PMID: 27584665. View Abstract on PubMed

Sant’Anna R, Navarro S, Ventura S, Paraoan L, Foguel D. 

Amyloid properties of the leader peptide of variant B cystatin C: implications for Alzheimer and Macular Degeneration. FEBS Lett. 2016 Feb 10. PMID: 26865059 View abstract on PubMed

Butler JM, Sharif U, Ali M, McKibbin M, Thompson JP, Gale R, Yang YC, Inglehearn C, Paraoan L.

A missense variant in CST3 exerts a recessive effect on susceptibility to age-related macular degeneration resembling its association with Alzheimer’s disease. Hum Genet. 2015 Apr 19. PMID: 25893795 View abstract on Pubmed

Hallstrom KN, Srikanth CV, Agbor TA, Dumont CM, Peters K, Paraoan L, Casanova JE, Boll EJ, McCormick BA.

PERP, a Host Tetraspanning Membrane Protein, is Required for Salmonella-Induced Inflammation. Cell Microbiol. 2014 Dec 9. PMID: 25486861 View abstract on Pubmed

Soriano-Romaní L, García-Posadas L, López-García A, Paraoan L, Diebold Y. 

Thrombospondin-1 induces differential response in human corneal and conjunctival epithelial cells lines under in vitro inflammatory and apoptotic conditions. Exp Eye Res. 2015 May;134:1-14. doi: 10.1016/j.exer.2015.03.004 View Abstract on Pubmed

Kay P, Yang YC, Hiscott P, Gray D, Maminishkis A, Paraoan L.

Age-related changes of cystatin C expression and polarized secretion by retinal pigment epithelium: potential age-related macular degeneration links. Invest Ophthalmol Vis Sci. 2014 Feb 14. PMID: 24458156 View abstract on Pubmed

Kay P, Yang YC, Paraoan L.

Directional protein secretion by the retinal pigment epithelium: roles in retinal health and the development of age-related macular degeneration. J Cell Mol Med. 2013 May 11. PMID: 23663427 View abstract on Pubmed

Davies L, Spiller D, White M R H, Grierson I, Paraoan L.

PERP expression stabilizes active p53 via modulation of p53-MDM2 interaction in uveal melanoma cells. Cell Death and Disease 2011 Mar 31; 2:e136. PMID: 21451571 View abstract on Pubmed

O’Reilly S, Pollock N, Currie L, Paraoan L, Clark AF, Grierson I. 

Inducers of cross-linked actin networks in trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2011 Sep 21;52(10):7316-24. doi: 10.1167/iovs.10-6692 View abstract on Pubmed

Paraoan L, Hiscott P, Gosden C, Grierson I.

Cystatin C in macular and neuronal degenerations: Implications for mechanism(s) of Age-related Macular Degeneration. Vision Res. 2010 mar 31;50(7):737-742. PMID: 19917302 View abstract on Pubmed

Davies L, Gray D, Spiller D, White MRH, Damato B, Grierson I, Paraoan L.

P53 apoptosis mediator PERP: localization, function and caspase activation in uveal melanoma. J Cell Mol Med. 2009 13;8B:1995-2007. PMID: 19040420 View abstract on Pubmed

Paraoan L, Gray D, Hiscott P, Garcia-Fianana M, Lane B, Damato B, Grierson I.

Cathepsin S and its inhibitor Cystatin C: imbalance in uveal melanoma. Frontiers in Bioscience. 2009 Jan 1;14:2504-2513. PMID: 19273215 View abstract on Pubmed

Paraoan L, Gray D, Hiscott P, Ebrahimi B, Damato B, Grierson I.

Expression of p53-induced apoptosis effector PERP in primary uveal melanomas: downregulation is associated with aggressive type. Exp Eye Res. 2006 Oct;83(4):911-9. PMID: 16784742 View Abstract on PubMed

Paraoan L, Grierson I.

Focus on molecules: Cystatin C. Exp Eye Res. 2007 Jun;84(6):1019-20. PMID: 16626703 View Abstract on PubMed

Ratnayaka A, Paraoan L, Spiller DG, Hiscott P, Nelson G, White MR, Grierson I.

A dual Golgi- and mitochondria-localised Ala25Ser precursor cystatin C: An additional tool for characterising intracellular mis-localisation leading to increased AMD susceptibility. Exp Eye Res. 2007 Jun;84(6):1135-9. PMID: 16635487 View Abstract on PubMed

Paraoan L, Grierson I, Maden BE.

Fate of cystatin C lacking the leader sequence in RPE cells. Exp Eye Res. 2003 Jun;76(6):753-6. PMID: 12742358 View Abstract on PubMed

Paraoan L, Grierson I, Maden BE.

Analysis of expressed sequence tags of retinal pigment epithelium: cystatin C is an abundant transcript. Int J Biochem Cell Biol. 2000 Apr;32(4):417-26. PMID: 10762067 View Abstract on PubMed

O’Reilly S, Pollock N, Currie L, Paraoan L, Clark AF, Grierson I.

Inducers of cross-linked actin networks in trabecular meshwork cells. Invest Ophthalmol Vis Sci. 2011 Sep 21;52(10):7316-24. PMID: 21849423 View Abstract on PubMed

Wade NC, Grierson I, O’Reilly S, Hoare MJ, Cracknell KP, Paraoan L, Brotchie D, Clark AF.

Cross-linked actin networks (CLANs) in bovine trabecular meshwork cells. Exp Eye Res. 2009;89(5):648-659. PMID: 19540832 View abstract on Pubmed

Cryan LM, Paraoan L, Hiscott P, Damato B, Grierson I, Gray D, Farrell M, Doherty GA, Fitzgerald DJ, O’Brien C.

Expression of COX-2 and prognostic outcome in uveal melanoma. Curr Eye Res. 2008 Mar 3;33:117-184. PMID: 18293189 View Abstract on Pubmed

Ratnayaka A, Paraoan L, Nelson G, Spiller DG, White MR, Hiscott P.

Trafficking of osteonectin by retinal pigment epithelial cells: evidence for basolateral secretion. Int J Biochem Cell Biol. 2007;39(1):85-92. PMID: 17035068 View Abstract on PubMed

Hiscott P, Paraoan L, Choudhary A, Ordonez JL, Al-Khaier A, Armstrong DJ.

Thrombospondin 1, thrombospondin 2 and the eye. Prog Retin Eye Res. 2006 Jan;25(1):1-18. PMID: 15996506 View Abstract on PubMed

Ordonez JL, Paraoan L, Hiscott P, Gray D, Garcia-Finana M, Grierson I, Damato B.

Differential expression of angioregulatory matricellular proteins in posterior uveal melanoma. Melanoma Res. 2005 Dec;15(6):495-502. PMID: 16314734 View Abstract on PubMed

Paraoan L, Ratnayaka A, Spiller DG, Hiscott P, White MR, Grierson I.

Unexpected intracellular localization of the AMD-associated cystatin C variant. Traffic. 2004 Nov;5(11):152-65. PMID: 15479453 View Abstract on PubMed

Hiscott P, Hagan S, Heathcote L, Sheridan CM, Groenewald CP, Grierson I, Wong D, Paraoan L.

Pathobiology of epiretinal and subretinal membranes: Possible roles for the matricellular proteins thrombospondin 1 and osteonectin (SPARC). Eye. 2002 Jul;16(4):393-403. PMID: 12101446 View Abstract on PubMed

Paraoan L, White MR, Spiller DG, Grierson I, Maden BE.

Precursor cystatin C in cultured retinal pigment epithelial cells: evidence for processing through the secretory pathway. Mol Membr Biol. 2001 Jul-Sep;18(3):229-36. PMID: 11681790 View Abstract on PubMed

Paraoan L, Grierson I, Maden BE.
Analysis of expressed sequence tags of retinal pigment epithelium: cystatin C is an abundant transcript.

Int J Biochem Cell Biol. 2000 32(4):417-26. doi: 10.1016/s1357-2725(99)00143-0.Abstract on PubMed: https://pubmed.ncbi.nlm.nih.gov/10762067/